中文名稱 :己糖磷酸激酶
其他中文名稱 :己糖激酶
英文名稱 : HK
其他英文名稱 : Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast
產(chǎn)品規(guī)格 : BR,~100u/mg
C A S :9001-51-8
級(jí) 別 :BR
分子量 :~ 54 kDa (monomer);~110 kDa (dimer)
活 力 :~ 100 units/mg protein
活力定義 :One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH :7.5~9.0
性 狀 :白色粉末。釀酒酵母提取。催化劑(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制劑(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用 途 :生化研究。為催化己糖6位上由ATP使之產(chǎn)生磷酸化反應(yīng)的酶。對(duì)α-D葡萄糖的親和性很高(Km=1×10-5M),對(duì)其他的己糖也可進(jìn)行磷酸化。此反應(yīng)的平衡向右偏移?!鱃°’=4.0千卡。該酶可被Mg2 、 K 所活化,但可被HS試劑阻抑。
保 存:-20℃